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1st group  (ATCC)
99
ATCC 1st group
1st Group, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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streptavidin  (Jackson Immuno)
96
Jackson Immuno streptavidin
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Streptavidin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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washing 0 avidinhrp  (Vector Laboratories)
86
Vector Laboratories washing 0 avidinhrp
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Washing 0 Avidinhrp, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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12-mg/h fentanyl patch  (Mylan Lab)
90
Mylan Lab 12-mg/h fentanyl patch
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
12 Mg/H Fentanyl Patch, supplied by Mylan Lab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rotary shaking model si4-2  (Sheldon Manufacturing)
90
Sheldon Manufacturing rotary shaking model si4-2
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Rotary Shaking Model Si4 2, supplied by Sheldon Manufacturing, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chicken anti-gfp gfp-1010  (AvesLabs)
90
AvesLabs chicken anti-gfp gfp-1010
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Chicken Anti Gfp Gfp 1010, supplied by AvesLabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gfp  (AvesLabs)
90
AvesLabs gfp
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Gfp, supplied by AvesLabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp/product/AvesLabs
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hcv 3.0 eia  (Quidel)
90
Quidel hcv 3.0 eia
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Hcv 3.0 Eia, supplied by Quidel, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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electrospinning machine nanospider® lab  (The Electrospinning Company Ltd)
90
The Electrospinning Company Ltd electrospinning machine nanospider® lab
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Electrospinning Machine Nanospider® Lab, supplied by The Electrospinning Company Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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12,12-dimethyl-10,12-dihydro-10-oxo- indeno[2,1-b]fluorene (98)  (Mylan Lab)
90
Mylan Lab 12,12-dimethyl-10,12-dihydro-10-oxo- indeno[2,1-b]fluorene (98)
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
12,12 Dimethyl 10,12 Dihydro 10 Oxo Indeno[2,1 B]Fluorene (98), supplied by Mylan Lab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lab12 qpcr high pure viral nucleic acid kit  (Roche)
86
Roche lab12 qpcr high pure viral nucleic acid kit
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Lab12 Qpcr High Pure Viral Nucleic Acid Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hcv ns5a  (Santa Cruz Biotechnology)
97
Santa Cruz Biotechnology hcv ns5a
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Hcv Ns5a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled streptavidin for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]

Journal: Purinergic Signalling

Article Title: A new role for the P2X7 receptor: a scavenger receptor for bacteria and apoptotic cells in the absence of serum and extracellular ATP

doi: 10.1007/s11302-012-9308-5

Figure Lengend Snippet: The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled streptavidin for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]

Article Snippet: Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega).

Techniques: Binding Assay, Incubation, Concentration Assay, Labeling