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1st group  (ATCC)
99
ATCC 1st group
1st Group, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated goat antihuman igg antibodies  (Vector Laboratories)
94
Vector Laboratories biotinylated goat antihuman igg antibodies
Biotinylated Goat Antihuman Igg Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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streptavidin  (Jackson Immuno)
96
Jackson Immuno streptavidin
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Streptavidin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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12-mg/h fentanyl patch  (Mylan Lab)
90
Mylan Lab 12-mg/h fentanyl patch
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
12 Mg/H Fentanyl Patch, supplied by Mylan Lab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rotary shaking model si4-2  (Sheldon Manufacturing)
90
Sheldon Manufacturing rotary shaking model si4-2
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Rotary Shaking Model Si4 2, supplied by Sheldon Manufacturing, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hcv 3.0 eia  (Quidel)
90
Quidel hcv 3.0 eia
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Hcv 3.0 Eia, supplied by Quidel, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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electrospinning machine nanospider® lab  (The Electrospinning Company Ltd)
90
The Electrospinning Company Ltd electrospinning machine nanospider® lab
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Electrospinning Machine Nanospider® Lab, supplied by The Electrospinning Company Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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12,12-dimethyl-10,12-dihydro-10-oxo- indeno[2,1-b]fluorene (98)  (Mylan Lab)
90
Mylan Lab 12,12-dimethyl-10,12-dihydro-10-oxo- indeno[2,1-b]fluorene (98)
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
12,12 Dimethyl 10,12 Dihydro 10 Oxo Indeno[2,1 B]Fluorene (98), supplied by Mylan Lab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hcv ns5a  (Santa Cruz Biotechnology)
97
Santa Cruz Biotechnology hcv ns5a
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Hcv Ns5a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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licosep-lab 12–26 1040  (Novasep Inc)
90
Novasep Inc licosep-lab 12–26 1040
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Licosep Lab 12–26 1040, supplied by Novasep Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nokia bell lab  (Nokia Corporation)
90
Nokia Corporation nokia bell lab
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Nokia Bell Lab, supplied by Nokia Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti adam19  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology rabbit anti adam19
The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled <t>streptavidin</t> for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]
Rabbit Anti Adam19, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled streptavidin for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]

Journal: Purinergic Signalling

Article Title: A new role for the P2X7 receptor: a scavenger receptor for bacteria and apoptotic cells in the absence of serum and extracellular ATP

doi: 10.1007/s11302-012-9308-5

Figure Lengend Snippet: The binding of short peptides mimicking P2X7 extracellular domain sequences to a range of phagocytic targets. 24 biotin-tagged peptides were incubated at a concentration of 10 μg/mL with either YG beads (3 μm, 5 μL), Alexa 488 conjugated Staphylococcus aureus or Escherichia coli (2 mg/mL, 5 μL), live S. aureus or E. coli (OD690 = 0.9, 10 μL each) or apoptotic HPB cells (5 × 106/mL, 200 μL) for 30 min, followed by washing and incubation with HRP-labeled streptavidin for 30 min. Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega). From Gu et al. [29]

Article Snippet: Particles were washed twice, and resuspended in 100 μL of HRP-labeled streptavidin (Jackson ImmunoResearch Lab, 1:2,000 diluted with PBS containing 1% BSA) for 30 min. After two washes, 500 μL of SuperSignal West Pico was added and the chemiluminescence was measured by a Glo 20/20 luminometer (Promega).

Techniques: Binding Assay, Incubation, Concentration Assay, Labeling